Assessment of the biotechnological activity of wheat hydrolysates prepared with the Biarum bovei extract

In this study, Biarum bovei extract was used to produce bioactive peptides from wheat gluten protein and the biological functional properties of hydrolysates were determinated. The results showed that has its highest protease activity (7.3 U/mg protein) at 45 °C pH 5. Based on electrophoresis analysis, molecular weight hydrolysate < 10 kDa. F1 fraction had antioxidant in DPPH (65.85 ± 2.64 µmol TE/g)) ABTS radical scavenging assays (295.81 TE/g). F2 with 86.3 0.48 ability inhibit ACE enzyme. F3 fractions statistically inhibition rate (49.37 0.12%. 79.19 1.13%) alpha-glucosidase alpha amylase, respectively. F1, an inhibitory effect Escherichia coli, Staphylococcus aureus, Listeria monocytogenes Bacillus cereus. Functional increasing weight, increased significantly. presence high levels (p ≤ 0.05) amino acids hydroxyl groups, hydrophobic positive charged critical role technological activity. These findings confirmed efficiency low (F1 3 kDa) biofunctionality such as activity, inhibitory, antidiabetic antibacterial could be beneficial health perspectives.